LabDisk meeting: Heraklion, Jul 18-19 2016
During the LabDisk Meeting that took place in Heraklion, Crete (Jul 18-19, 2016) the following main points were agreed by all participants:
- Two separate disks will be developed: one for A. gambiae assays, one for A. funestus assays. Simultaneous DNA and RNA extraction will be made on-disk.
- A total of 12 qPCR reaction chambers will be used. Detection of 3 colors in each reaction chamber. Adding a fourth color/probe is also possible.
- Assays will be firstly developed in individual mosquito strains (optimization from the analytical point of view) and then in mosquito pools (optimization from the diagnostic point of view).
- DDMS: Output from LabDisk should be able to be exported as raw data (Ct and/or endpoint fluorescence values) in csv format, data should be then inputted in a stand-alone simple excel file. After transformations and calculations data will be entered to the DDMS.